NDUFA2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O43678

Core Product Specifications and Parameters

Biological Background: NDUFA2 Function and Localization

• NDUFA2 encodes the NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2, also known as Complex I-B8 (CI-B8) or the B8 subunit—a key accessory component of mitochondrial respiratory chain Complex I.
• As a non-catalytic accessory subunit, NDUFA2 is essential for the structural integrity and assembly of Complex I, which catalyzes the transfer of electrons from NADH to ubiquinone in the electron transport chain.
• The protein is anchored to the mitochondrial inner membrane, consistent with its role in oxidative phosphorylation and cellular energy metabolism.
• NDUFA2 undergoes alternative splicing, and its mature form is subject to post-translational modifications such as acetylation and disulfide bond formation, which may influence its function or stability.
• Dysfunctional NDUFA2 variants are linked to primary mitochondrial disease (e.g., MC1DN13), underscoring its clinical importance in metabolic and neurodegenerative disorders.
• With a molecular weight of approximately 11 kDa, NDUFA2 is identified in high-throughput proteomics studies as a reference proteome member.
• Key associated biological processes include electron transport, respiratory chain, and transport; the protein also features documented 3D structures.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human NDUFA2 protein (aa 1–95), suggesting the antibody recognizes the native and denatured forms; verify performance in your specific assay conditions.
• For Western blot, a band near 11 kDa is expected in human, mouse, and rat samples; consider using reduced/non-reduced conditions to assess disulfide-bonded states.
• In immunofluorescence/ICC, anticipate a mitochondrial staining pattern; co-staining with organelle markers is recommended to confirm specificity.
• For ELISA, the polyclonal nature provides good sensitivity; however, batch-to-batch consistency should be validated for quantitative applications.
• As the antibody is shipped on ice bags, store aliquots at -20 °C or below upon receipt; avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing bridge for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and custom rare-target development services. Our NDUFA2 rabbit polyclonal antibody supports mitochondrial and metabolic disease research, complementing our portfolio of respiratory chain and oxidative phosphorylation reagents. Whether you need research-grade antibodies, large-scale production, or technical consulting from concept to clinic, CamelBio is your trusted partner.