NDP Rabbit pAb - Q00604

Core Product Specifications and Parameters

Biological Background: Norrin Function and Localization

• Norrin (also known as Norrie disease protein or X-linked exudative vitreoretinopathy 2 protein) is a secreted protein encoded by the NDP gene in humans.
• It functions as a ligand for the FZD4/LRP5 receptor complex, activating the canonical Wnt signaling pathway to stabilize β-catenin and promote LEF/TCF-mediated transcription.
• Norrin plays a critical role in retinal vascularization, with mutations linked to Norrie disease and familial exudative vitreoretinopathy (FEVR).
• The protein acts in concert with TSPAN12 to activate FZD4 independently of traditional Wnt ligands, indicating a Wnt-independent signaling mechanism.
• Subcellularly, Norrin is secreted and found in the extracellular space.
• It is primarily expressed in the retina (outer nuclear, inner nuclear, and ganglion cell layers) and in fetal and adult brain tissues.
• Key structural features include disulfide bonds and a signal peptide, with a molecular weight of approximately 15 kDa.
• Additional keywords indicate involvement in sensory transduction, vision, and deafness, as well as being a reference proteome component with known 3D structures.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant protein spanning amino acids 25-133 of human Norrin. Consider that the polyclonal antibody may recognize multiple epitopes within this region.
• For Western blotting, a band around 15 kDa is expected; validate the antibody in human retinal or brain tissue lysates.
• In ELISA, the antibody may be suitable for detecting recombinant or secreted Norrin; consider optimizing coating conditions and using appropriate negative controls.
• Since Norrin is a secreted protein, samples such as cell culture supernatants or biological fluids may require concentration before analysis.
• Cross-reactivity is reported for human; if using in other species, perform sequence alignment and validation experiments.

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