NCS1 Rabbit Monoclonal Antibody for WB and ELISA - P62166

Core Product Specifications and Parameters

Biological Background: Neuronal Calcium Sensor 1 Function and Localization

• Neuronal calcium sensor 1 (NCS1), also known as frequenin homolog, is a member of the neuronal calcium sensor family of EF-hand calcium-binding proteins.
• Acts as a calcium-dependent regulator of G protein-coupled receptor phosphorylation, specifically regulating rhodopsin kinase (GRK1) but not GRK2–GRK5.
• Stimulates phosphatidylinositol 4-kinase beta (PI4KB) kinase activity, implicating it in phosphoinositide signaling.
• Involved in long-term synaptic plasticity through interaction with protein interacting with C kinase 1 (PICK1).
• May play a role in neuronal differentiation by inhibiting N-type voltage-gated calcium channels.
• Localizes to the Golgi apparatus, postsynaptic density, cytoplasm (perinuclear region), cell membrane, and possibly other membrane compartments.
• Post-translationally modified by N-terminal myristoylation (lipoprotein) and contains multiple EF-hand domains for calcium binding.
• The gene undergoes alternative splicing and is classified under pharmaceutical relevance, with identified proteomics expression.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is 22 kDa; ensure sufficient resolution on SDS-PAGE gels and use appropriate loading controls.
• Validate antibody reactivity in human, mouse, and rat neuronal cell lines or tissue lysates; consider using brain or neural-derived samples.
• For ELISA, the synthetic peptide immunogen (aa 91–190) may be used as coating antigen; indirect ELISA formats are typically suitable.
• Because the epitope lies within a structured region, confirm target detection in native protein conformations under non-denaturing conditions if required.
• Optimize blocking and antibody dilution conditions for each assay to minimize background, especially when using tissue lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with efficient access to high-quality IVD raw materials for neuroscience and calcium signaling targets such as NCS1. Our one-stop platform streamlines procurement of validated monoclonal antibodies, antigen-grade peptides, and ancillary reagents—allowing you to focus on assay development without supply chain disruptions. Whether you need bulk quantities of this anti-NCS1 mAb or custom sourcing for rare targets, we connect you with trusted resources to move from concept to clinic.