NCR3 Rabbit pAb for WB, ELISA - O14931

Core Product Specifications and Parameters

Biological Background: NCR3 Function and Localization

• NCR3 (Natural cytotoxicity triggering receptor 3), also known as NKp30 or CD337, is an activating natural killer (NK) cell receptor encoded by the NCR3 gene.
• It functions as a cell membrane receptor that triggers NK cell cytotoxicity upon binding to extracellular ligands such as BAG6 and NCR3LG1, promoting killing of tumor cells.
• Engagement of NCR3 by its ligand BAG6 also promotes myeloid dendritic cell (DC) maturation, both by eliminating immature DCs and by inducing NK cell release of TNF-α and IFN-γ.
• The receptor is selectively expressed on the surface of all resting and activated NK cells, with weak expression detected in the spleen.
• As a type I transmembrane glycoprotein, NCR3 contains an immunoglobulin-like domain in its extracellular region and relies on associated adaptor proteins (e.g., CD3ζ, FcεRIγ) for signal transduction.
• Post-translational modifications include N-linked glycosylation and formation of a disulfide bond within the Ig domain, which are critical for proper folding and ligand recognition.
• Alternative splicing generates multiple isoforms, adding to the functional diversity of the receptor in immune surveillance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 19-135), which includes the ligand-binding Ig-like region; therefore, the antibody is expected to recognize the native receptor on the cell surface.
• For Western blotting under reducing conditions, consider a loading amount of 20-40 µg of lysate from NK cell lines (e.g., NK-92) or activated primary NK cells, and validate the band at the predicted molecular weight (~22 kDa).
• Due to glycosylation and potential isoform variation, the observed molecular weight in WB may appear slightly higher; inclusion of deglycosylation controls or use of specific cell lines can help verify specificity.
• In ELISAs, coating with recombinant NCR3 protein or cell-based formats can be explored; appropriate blocking and detection conditions should be optimized for the specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw material sourcing, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to support your NK cell-targeted diagnostic development. With a focus on rare and critical targets like NCR3, we facilitate access to high-quality raw materials and technical support to accelerate your project from concept to clinic. Reach out to explore how our integrated supply solutions can meet your specific procurement needs.