NCOA4 Rabbit Monoclonal Antibody for WB, IP, ELISA - Q13772

Core Product Specifications and Parameters

Biological Background: NCOA4 Function and Localization

• NCOA4 (nuclear receptor coactivator 4), also known as ARA70, ELE1, and RFG, is a multifunctional protein with isoforms including a testis-specific isoform Beta.
• As a cargo receptor for ferritinophagy, NCOA4 targets the iron-binding ferritin complex to autolysosomes upon starvation or iron depletion, playing a central role in cellular iron homeostasis. Related references: PMID:25327288 PMID:26436293
• NCOA4 contributes to efficient erythropoiesis, potentially by modulating hemin-induced erythroid differentiation. Related references: PMID:26436293
• The protein has been reported to enhance androgen receptor (AR) transcriptional activity and to act as a ligand-independent coactivator of peroxisome proliferator-activated receptor gamma (PPARγ), though some studies suggest only weak coactivator activity for AR. Related references: PMID:10347167 PMID:8643607 PMID:10517667
• NCOA4 binds to DNA replication origins in a transcription-independent manner and may inhibit origin activation through interaction with the MCM2-7 helicase complex. Related references: PMID:24910095
• Subcellularly, NCOA4 localizes to cytoplasmic vesicles, autophagosomes, autolysosomes, the nucleus, and chromosomes, consistent with its roles in autophagy and gene regulation.
• Widely expressed across tissues, with the Beta isoform restricted to testis, NCOA4 is involved in key processes including iron metabolism and transcriptional regulation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide; thus, the antibody is expected to recognize linear epitopes and is ideal for denaturing Western blot analysis.
• For immunoprecipitation (IP), validate antibody performance under native conditions, as epitope accessibility may vary.
• For ELISA, the antibody may be used as a capture or detection reagent; optimization of coating and detection conditions is recommended.
• Cross-reactivity has been demonstrated with human, mouse, and rat samples; always include appropriate controls when working with these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined sourcing experience for IVD raw materials. This NCOA4 monoclonal antibody supports research into ferritinophagy, iron homeostasis, and nuclear receptor coactivator biology. Beyond this product, we supply validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare-target raw materials to accelerate your assay development from concept to clinic.