NCAPD2 Polyclonal Antibody for WB and ELISA - Q15021

Core Product Specifications and Parameters

Biological Background: NCAPD2 (Condensin Complex Subunit 1) Function and Localization

• NCAPD2, also known as condensin complex subunit 1, hCAP-D2, or chromosome-associated protein D2, is a regulatory subunit of the condensin I complex.
• The condensin complex mediates mitotic chromosome condensation by introducing positive supercoils into DNA, a process essential for proper chromosome segregation (PubMed:11136719). Related references: PMID:11136719
• NCAPD2 may target the condensin complex to DNA through its C-terminal domain and promotes resolution of DNA catenanes between sister chromatids.
• It is required for decatenation of non-centromeric ultrafine DNA bridges during anaphase and plays a critical role in neurogenesis by ensuring accurate chromosome condensation in neural stem cells, affecting cortex size (PubMed:27737959). Related references: PMID:27737959
• Subcellular localization is primarily nuclear, with presence in cytoplasm and on chromosomes during mitosis.
• The protein is subject to phosphorylation and is associated with DNA condensation, mitosis, and cell division; disease variants link NCAPD2 to primary microcephaly.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 1250–1350), suggesting the antibody may recognize full-length NCAPD2 in denaturing Western blot. For ELISA, consider a peptide-based assay setup.
• Validate specificity in relevant cell lines (human, mouse, rat) given the stated cross-reactivity; include appropriate loading controls due to the large target size (~157 kDa).
• For Western blot, use a sufficiently high-percentage gel or extended transfer times to ensure efficient transfer of this high molecular weight protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as a comprehensive sourcing partner for IVD raw materials, supporting your chromosome biology and cell division research. We offer validated antibodies like this anti-NCAPD2 polyclonal antibody, along with ancillary reagents and bulk raw material supply, to streamline your assay development from concept to clinic.