NAT2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P11245

Core Product Specifications and Parameters

Biological Background: NAT2 Function and Localization

• NAT2, also known as arylamine N-acetyltransferase 2, arylamide acetylase 2, and polymorphic arylamine N-acetyltransferase (PNAT), is encoded by the NAT2 gene (also known as AAC2) in humans.
• Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates, playing a critical role in the metabolism of xenobiotics. Related references: PMID:12222688 PMID:7915226
• Participates in the detoxification of numerous hydrazine and arylamine drugs, and bioactivates several known carcinogens, making it a key determinant in pharmacogenomic variability.
• It is a member of the arylamine N-acetyltransferase family; NAT2 is the primary polymorphic form responsible for the classic rapid vs slow acetylator phenotype.
• Subcellularly, NAT2 is localized to the cytoplasm, consistent with its role in xenobiotic metabolism.
• The mature protein has a calculated molecular weight of approximately 34 kDa (33.571 kDa in UniProt) and is classified under acyltransferase and transferase families.
• Keywords in UniProt include 3D-structure, direct protein sequencing, and proteomics identification, reflecting available structural and proteomic data.
• NAT2 acetylation activity is critical for drug metabolism, carcinogen detoxification, and cancer susceptibility, with polymorphisms influencing individual responses.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 171–290) of human NAT2, which may confer specificity for NAT2 over closely related isoforms like NAT1; validate in your experimental system if isoform distinction is critical.
• As a rabbit polyclonal antibody, it recognizes multiple epitopes, potentially reducing sensitivity to minor sequence variations; however, for quantitative studies, consider assessing specificity with recombinant NAT2 and NAT1 proteins.
• The antibody is specified for WB, IHC-P, and ELISA. For each application, optimize dilutions and detection conditions using appropriate positive and negative controls (e.g., NAT2-overexpressing lysates, pharmacologically relevant tissue sections).
• Given the highly polymorphic nature of NAT2, the antibody may not recognize all allelic variants equally; sequence verification of the target sample is recommended, especially in functional pharmacological assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined, one-stop access to validated IVD raw materials, including this NAT2 antibody for drug metabolism, carcinogen bioactivation, and pharmacogenomics research. We support procurement of bulk ancillary reagents and rare-target antibody sourcing to accelerate your assay development from concept to clinical application. Explore our comprehensive portfolio to enhance your biomarker research and in vitro diagnostic programs.