NAT10 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H0A0

Core Product Specifications and Parameters

Biological Background: NAT10 Function and Localization

• RNA cytidine acetyltransferase that catalyzes formation of N(4)-acetylcytidine (ac4C) on mRNAs, 18S rRNA, and tRNAs. Related references: PMID:25411247, PMID:25653167, PMID:30449621
• Enhances mRNA stability and translation through ac4C modification, often within wobble cytidine sites. Related references: PMID:30449621, PMID:35679869
• Mediates ac4C at position 1842 in 18S rRNA and is required for early nucleolar cleavages during 18S rRNA synthesis. Related references: PMID:25411247, PMID:25653167
• Also exhibits in vitro lysine acetyltransferase activity toward proteins like histones, microtubules, p53, and MDM2; however, the in vivo relevance is uncertain. Related references: PMID:14592445, PMID:17631499, PMID:19303003, PMID:30449621
• Activates telomerase by stimulating TERT transcription and may influence telomerase subunit assembly. Related references: PMID:14592445, PMID:18082603
• Regulates centrosome duplication by acetylating CENATAC during mitosis, promoting SASS6 proteasome degradation. Related references: PMID:31722219
• Component of the small subunit (SSU) processome, involved in early ribosome biogenesis and pre-rRNA processing. Related references: PMID:34516797
• Subcellular localization includes the nucleolus, nucleus, and midbody; subject to alternative splicing, ATP-binding, and phosphorylation (UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1–250 amino acids of human NAT10, which may target regions critical for RNA binding or catalytic activity; consider validating the antibody in your system.
• Suitable applications include Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA. For WB, a band around 116 kDa is expected for human NAT10; verify with a positive control.
• Human and rat cross‑reactivity are supported; for other species, sequence homology should be checked before use.
• In IF/ICC, NAT10 localizes to nucleoli and the nucleus, with possible midbody staining during cell division; optimize fixation and permeabilization accordingly.
• As a polyclonal antibody, lot‑to‑lot consistency should be monitored; consider including a known reference standard in each experiment.
• For functional studies, note that NAT10 acetylates RNA and proteins; appropriate controls (e.g., ac4C modification assays or siRNA knockdown) may help confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one‑stop access to high‑quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like NAT10—an emerging biomarker and drug target in cancer and RNA modification research—we offer sourcing solutions that span from concept to clinic. Whether you need established reagents or support with rare‑target raw‑material procurement, CamelBio bridges your R&D needs with scalable supply.