NAPSA Rabbit Polyclonal Antibody for WB, IF-P, ELISA - O96009

Core Product Specifications and Parameters

Biological Background: NAPSA Function and Localization

• NAPSA encodes napsin-A, an aspartyl protease also known as ASP4, napsin-1, or TA01/TA02.
• Napsin-A is a secreted zymogen that requires proteolytic cleavage for activation, and contains disulfide bonds and glycosylation sites.
• It may be involved in the processing of pneumocyte surfactant precursors, suggesting a role in lung function.
• Expressed predominantly in adult lung type II pneumocytes and kidney, with lower levels in spleen and peripheral blood leukocytes.
• Subcellular localization is secreted, consistent with its function in extracellular substrate processing.
• Classified as a hydrolase (protease) within the aspartyl protease family.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region of human NAPSA (aa 321-420), which may confer specificity for the mature or zymogen form; sequence alignment with mouse and rat orthologs is advised to assess cross-reactivity.
• For WB, the predicted molecular weight is 45 kDa; consider using reducing conditions and validate with positive controls such as lung or kidney lysates.
• In IF-P, permeabilization may be necessary to detect intracellular forms, but secreted NAPSA staining may require optimized fixation protocols.
• Cross-reactivity is indicated for mouse and rat; however, epitope homology should be verified experimentally in your sample system.
• Due to its role as a protease zymogen, activation status in tissues may influence detection; consider testing under various biological conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a complete IVD raw material supply chain, from validated antibody pairs and polyclonal reagents to bulk ancillary consumables, tailored for diagnostic and research applications. For lung biology or protease-focused projects, we can source rare-target antibodies like anti-NAPSA, supporting your transition from concept to clinical development. Our technical consulting and one-stop platform streamline procurement, ensuring timely access to critical reagents.