NAIF1 Rabbit pAb - Q69YI7

Core Product Specifications and Parameters

Biological Background: NAIF1 Function and Localization

• Nuclear apoptosis-inducing factor 1 (NAIF1), also known as C9orf90, is a pro-apoptotic factor encoded by the NAIF1 gene.
• Its primary function is to induce apoptosis, as indicated by functional characterization.
• The protein localizes exclusively to the nucleus.
• NAIF1 is widely expressed in human tissues, suggesting a fundamental role in cellular homeostasis.
• Multiple isoforms are generated through alternative splicing.
• Keywords: Apoptosis, Nucleus, Alternative splicing, Reference proteome, Proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-110), which may be important for antibody specificity; verify epitope conservation in target species.
• For Western blot (WB), the predicted molecular weight is approximately 35 kDa; use suitable gel percentages (e.g., 10–12% SDS-PAGE) for optimal separation.
• Since NAIF1 is widely expressed, many cell lines (e.g., HeLa, HEK293) may serve as positive controls; however, validate expression in the relevant sample system.
• For ELISA, titrate the antibody and optimize coating/blocking conditions according to standard polyclonal antibody protocols.
• Consider that polyclonal antibodies may exhibit batch-to-batch variability; perform lot-specific validation for critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing for research involving nuclear apoptosis factors like NAIF1. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to support your assay development. For rare target sourcing or custom requirements, we provide tailored procurement solutions to accelerate your project from concept to clinic.