NAF1 Rabbit Polyclonal Antibody for WB, ELISA - Q96HR8

Core Product Specifications and Parameters

Biological Background: NAF1 Function and Localization

• NAF1 is an RNA-binding protein and a non-core subunit of the H/ACA ribonucleoprotein (snoRNP) complex, essential for ribosome biogenesis.
• It functions in the maturation of box H/ACA snoRNPs by transiently associating with the complex; it is later replaced by NOLA1/GAR1 to form the mature snoRNP, possibly competing for binding with DKC1/NOLA4.
• The protein localizes to both the cytoplasm and nucleus, consistent with its role in snoRNP assembly and transport.
• NAF1 undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitin-like conjugation (isopeptide bond), which may regulate its activity.
• Alternative splicing of the NAF1 gene produces multiple transcript variants.
• It is identified as a reference proteome component and is involved in rRNA processing.
• The molecular weight of the canonical isoform is approximately 53.7 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 150–400 of human NAF1, a region conserved in mouse and rat, supporting the observed cross-reactivity. Consider using this antibody for WB in lysates from human, mouse, or rat cells.
• For ELISA, the recombinant protein immunogen can serve as a positive control to validate assay conditions.
• Since NAF1 is a low-abundance protein involved in ribosome biogenesis, enrichment of nuclear or cytoplasmic fractions may improve detection sensitivity in WB.
• Validate antibody specificity by comparing signal in NAF1-overexpressing versus knockdown samples.
• The recommended molecular weight for NAF1 is ~54 kDa; use appropriate molecular weight markers for verification.

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CamelBio provides diagnostic manufacturers and researchers with a comprehensive range of IVD raw materials, including validated antibodies like this anti-NAF1 polyclonal antibody, for ribosome biogenesis and cancer biomarker studies. Beyond catalog products, we offer custom antibody development, bulk reagent supply, and rare target sourcing to accelerate your assay development from concept to clinic.