NAA60 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9H7X0

Core Product Specifications and Parameters

Biological Background: NAA60 Function and Localization

• NAA60 (also known as NatF, HAT4) is an N-alpha-acetyltransferase that specifically acetylates the N-terminal residues of transmembrane proteins, with a strong preference for N-termini facing the cytosol. Related references: PMID:25732826 PMID:38480682
• It displays N-terminal acetyltransferase activity towards sequences starting with Met-Lys, Met-Val, Met-Ala, and Met-Met. Related references: PMID:21750686 PMID:25732826 PMID:27320834
• NAA60 is required for normal chromosomal segregation during anaphase. Related references: PMID:21750686
• The protein localizes to the Golgi apparatus membrane.
• Alternative splicing and alternative promoter usage generate multiple isoforms of NAA60.
• It may also exhibit histone acetyltransferase activity in vitro, though the in vivo relevance remains to be fully established. Related references: PMID:21981917
• Keywords associated with NAA60 include acetylation, acyltransferase, chromatin regulator, and chromosome partition; it belongs to the reference proteome with a predicted molecular weight of 27 kDa.

Experimental Guidance and Technical Tips

• The immunogen used to generate this rabbit polyclonal antibody spans the N-terminal 1-184 amino acids of human NAA60, a region that includes the N-acetyltransferase domain.
• In Western blot (WB), expect a primary band around 27 kDa; additional bands may appear due to alternative splicing isoforms or post-translational modifications.
• For immunohistochemistry (IHC-P), appropriate antigen retrieval methods and validation with positive and negative tissue controls are recommended.
• Cross-reactivity is reported for human, mouse, and rat; verify expression in your sample system and consider using species-specific controls.
• As a polyclonal antibody, lot-to-lot variability may occur; it is advisable to test each new lot in pilot experiments before large-scale use.
• ELISA applications may require optimization of antibody concentration and coating conditions for robust signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, purified antibodies, and ancillary reagents, supporting development from concept to clinical application. This NAA60 polyclonal antibody, targeting a key enzyme in protein acetylation and chromosomal regulation, is well-suited for studies in cancer biology, epigenetics, and cell cycle control. For bulk orders, customized sourcing, or technical consultation, our team is ready to streamline your procurement process.