MYOT Rabbit pAb for WB, IF/ICC, ELISA - Q9UBF9

Core Product Specifications and Parameters

Biological Background: Myotilin Function and Localization

• Myotilin is a 57 kDa cytoskeletal protein also known as myofibrillar titin-like Ig domains protein or titin immunoglobulin domain protein, encoded by the MYOT (TTID) gene.
• It serves as a component of a multi-protein complex involved in actin cross-linking, playing a critical role in the control of myofibril assembly and stability at the Z lines in muscle cells.
• The protein localizes to the cell membrane (sarcolemma), cytoplasm, cytoskeleton, and specifically to the myofibril sarcomere Z line, indicating its integral role in muscle ultrastructure.
• Tissue expression is predominantly in skeletal muscle (at the protein level), with additional expression in heart, bone marrow, and thyroid gland, suggesting specialized functions in striated and non-striated tissues.
• Myotilin contains immunoglobulin-like domains and repeat regions, consistent with its structural role in sarcomere organization, and is subject to post-translational modifications including methylation.
• Mutations in MYOT are associated with disease variants such as limb-girdle muscular dystrophy and myofibrillar myopathy, underscoring its importance in muscle integrity.
• Alternative splicing generates multiple isoforms, which may contribute to functional diversity in different muscle types or developmental stages.

Experimental Guidance and Technical Tips

• The antibody has been produced against a recombinant protein containing amino acids 259–314 of human myotilin; consider validating specificity in your own sample system using appropriate controls.
• For Western blotting, the expected band is around 55 kDa; ensure adequate membrane blocking and optimize primary antibody dilution for your specific lysate.
• Immunofluorescence/immunocytochemistry may be used to study myotilin localization in muscle cells; co-staining with Z line markers is recommended.
• ELISA protocols can be established using the antibody as a capture or detection reagent; consider testing with recombinant myotilin or tissue extracts.
• Cross-reactivity with mouse and rat has been reported; however, perform a species-specific validation if using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of IVD raw materials for diagnostic developers and researchers, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. For studies focused on muscle biology, myofibrillar proteins, or neuromuscular disorders, CamelBio can supplement your project with complementary antibodies, antigens, and technical support. Connect with us to efficiently bridge your concept-to-clinic needs with reliable material supply.