MYOM2 Rabbit Polyclonal Antibody for WB, ELISA - P54296

Core Product Specifications and Parameters

Biological Background: MYOM2 Function and Localization

• Myomesin-2 (MYOM2), also designated 165 kDa connectin/titin-associated protein or M-protein, belongs to the myomesin family of sarcomeric proteins.
• It is a major structural component of the M band in vertebrate striated muscle myofibrils, where thick filaments are cross-linked.
• MYOM2 mediates the anchoring of myosin thick filaments to the elastic titin scaffold at the M line through direct, dose-dependent binding interactions.
• The protein localizes exclusively to the cytoplasm, specifically within the sarcomeric M line of mature myofibrils.
• Structurally, MYOM2 contains multiple immunoglobulin-like domains and other repeat motifs that facilitate its interaction with myosin and titin.
• As an essential muscle protein, MYOM2 contributes to maintaining sarcomere integrity and ensuring proper contractile function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-200) of human MYOM2; consider using full-length protein controls to assess antibody specificity.
• For Western blotting, the predicted molecular weight of MYOM2 is approximately 165 kDa; use appropriate molecular weight markers and transfer conditions for high-molecular-weight proteins.
• Given the polyclonal nature of this antibody, validate reactivity in the relevant species (human, mouse, rat) and tissue type (e.g., skeletal or cardiac muscle lysates).
• ELISA experiments may be optimized using coated MYOM2 recombinant protein or peptide fragments; titrate antibody concentrations to determine optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a streamlined one-stop platform for IVD raw material procurement. This MYOM2 polyclonal antibody supports myofibrillar M band and sarcomere biology investigations. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary supplies, and custom rare-target sourcing to accelerate your assay development from concept to clinic.