Myoglobin Rabbit Monoclonal Antibody for WB and ELISA - P02144

Core Product Specifications and Parameters

Biological Background: Myoglobin Function and Localization

• Myoglobin is a monomeric heme protein primarily responsible for oxygen storage and facilitated diffusion in muscle tissues (PubMed:30918256, PubMed:34679218).

  Related references: PMID:30918256 PMID:34679218

• It exhibits nitrite reductase activity, regulating the production of bioactive nitric oxide under certain oxidative conditions (PubMed:32891753).

  Related references: PMID:32891753

• Under hypoxic or anoxic stress, Myoglobin protects cells against reactive oxygen species via its pseudoperoxidase activity (PubMed:34679218).

  Related references: PMID:34679218

• The protein localizes predominantly to the cytoplasm and sarcoplasm of muscle cells.

• Myoglobin contains a heme prosthetic group and binds iron, which are essential for oxygen transport and enzymatic activities.

• Known as Nitrite reductase MB or Pseudoperoxidase MB, reflecting its alternative catalytic functions.

• It is a phosphoprotein and has been associated with disease variants, highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide spanning amino acids 55-154 of human Myoglobin; consider this region when designing detection assays.
• This antibody is validated for Western blot (WB) and ELISA applications; for other techniques like IHC or IF, validation in your specific experimental system is recommended.
• Given the cross-reactivity with mouse and rat, the antibody may be suitable for detecting Myoglobin in these species, but it is advisable to verify cross-reactivity in the relevant sample context.
• Myoglobin has a predicted molecular weight of ~17 kDa; optimize SDS-PAGE gel percentage (e.g., 12-15%) for effective separation in WB.
• For ELISA, consider pairing this antibody with a matched detection antibody, as antibody pair optimization remains critical for assay sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing platform for IVD manufacturers and research laboratories, offering a wide range of Myoglobin-related raw materials—including validated monoclonal antibodies, antibody pairs, and bulk ancillary reagents. Whether advancing muscle oxygen transport studies or developing diagnostic assays for myoglobin-related disorders, CamelBio streamlines procurement of high-quality, rare-target raw materials from concept to clinic.