MyoD1 Rabbit pAb for WB and ELISA - P15172

Core Product Specifications and Parameters

Biological Background: MyoD1 Function and Localization

• MyoD1 (Myoblast determination protein 1), also known as MYF3 or bHLHc1, is a muscle-specific transcription factor that plays a key role in muscle differentiation.
• It acts as a transcriptional activator, promoting the expression of muscle-specific target genes during myogenesis.
• MyoD1 cooperates with related myogenic factors MYF5 and MYOG to co-occupy promoter regions of muscle-specific genes, driving the myogenic program.
• The protein induces the conversion of fibroblasts into myoblasts, demonstrating its master regulatory role in muscle cell fate determination.
• MyoD1 activity is negatively regulated through direct interaction with the twist protein, likely involving their basic domains.
• It localizes exclusively to the nucleus, where it binds DNA to regulate transcription.
• Post-translational modifications on MyoD1 include acetylation, phosphorylation, methylation, and ubiquitin-like conjugation, which modulate its activity and stability.
• Keywords associated with MyoD1 include DNA-binding, developmental protein, myogenesis, and disease variants, highlighting its importance in muscle development and pathology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 226-300) of human MyoD1, which may enhance specificity in detection applications.
• For Western Blot, a band near 35 kDa is expected in muscle cell lines or myogenic samples; validate with appropriate positive controls.
• In ELISA, consider using the recombinant immunogen as a standard or coating antigen for optimal performance.
• Due to mouse cross-reactivity, ensure mouse samples are properly validated in your specific experimental context.
• As a polyclonal antibody, lot-to-lot consistency should be monitored; performing titration for each new lot is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw material sourcing, from early-stage research to clinical application. This MyoD1 antibody supports muscle differentiation studies and myogenesis research, complementing our extensive portfolio of validated antibodies, recombinant proteins, and ancillary reagents. For specialized requirements—whether optimized antibody pairs, bulk polyclonal antibodies, or rare target reagents—CamelBio’s expert team connects you with reliable, high-quality solutions to streamline your diagnostic assay development.