MYO5A Rabbit Polyclonal Antibody for WB, ELISA - Q9Y4I1

Core Product Specifications and Parameters

Biological Background: MYO5A Function and Localization

• Unconventional myosin-Va, also known as dilute myosin heavy chain, MYH12, or myoxin, is encoded by the MYO5A gene.
• Functions as an actin-based motor protein that hydrolyzes ATP and moves processively in ~36-nm steps along actin filaments (PubMed:10448864). Related references: PMID:10448864
• Involved in melanosome transport within melanocytes, contributing to pigmentation.
• Mediates transport of vesicles to the plasma membrane and may play a role in dendrite polarization during neuronal development (by similarity).
• Contains actin-binding motor domain, calmodulin-binding IQ motifs, coiled-coil region, and a C-terminal globular tail domain.
• Subject to post-translational modifications including phosphorylation and acetylation, which may regulate activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen targets residues 950–1150 of human MYO5A; for WB, expect a band near 215 kDa and consider protease inhibitors due to large protein size.
• For ELISA, use recombinant MYO5A protein or peptide as coating antigen and include appropriate controls to verify specificity.
• Validate in relevant samples such as melanocytes or neuronal cells, and confirm cross-reactivity if using mouse or rat models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to IVD raw materials, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support your MYO5A research and diagnostic development. Streamline your supply chain and accelerate from concept to clinic with CamelBio’s integrated procurement and technical solutions.