MYLK3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q32MK0

Core Product Specifications and Parameters

Biological Background: MYLK3 Function and Localization

• MYLK3, also known as Cardiac-MyBP-C-associated Ca/CaM kinase (Cardiac-MLCK), is a serine/threonine-protein kinase belonging to the myosin light chain kinase family.
• It phosphorylates myosin regulatory light chain 2 (MYL2) in vitro, a role crucial for cardiac muscle contraction regulation (By similarity).
• MYLK3 promotes sarcomere formation in cardiomyocytes and enhances cardiomyocyte contractility, highlighting its importance in cardiac muscle structure and function (By similarity).
• The kinase is predominantly expressed in heart tissue, as indicated by its restricted tissue specificity.
• MYLK3 localizes to the cytoplasm, where it interacts with components of the cardiac sarcomere.
• It is classified under several functional keywords, including ATP-binding, magnesium, nucleotide-binding, kinase, serine/threonine-protein kinase, and transferase, reflecting its enzymatic activity.
• UniProt annotates MYLK3 as subject to alternative splicing and phosphoprotein modifications, suggesting regulatory diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100–200 of human MYLK3. For Western blot, consider using cardiac tissue lysates (human or mouse) as positive controls to detect an approximately 88 kDa band.
• For IF/ICC, validate localization in cardiomyocyte cell lines or primary cardiac tissue sections, anticipating cytoplasmic staining.
• In ELISA, the antibody may be used for detecting recombinant or native MYLK3; optimize coating and detection conditions with appropriate controls.
• Note that MYLK3 is expressively restricted to heart; use heart-derived samples for relevant applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with one-stop access to high-quality IVD raw materials, including validated antibodies like this anti-MYLK3 polyclonal antibody. For projects targeting cardiac kinase signaling or sarcomere biology, we can also source monoclonal antibodies, antigen pairs, and bulk ancillary reagents. Contact us to explore custom raw-material solutions tailored to your assay development needs.