MYL6B Rabbit pAb for WB, ELISA - P14649

Core Product Specifications and Parameters

Biological Background: MYL6B Function and Localization

• MYL6B, also named MLC1SA or smooth muscle and nonmuscle myosin light chain alkali 6B, is a regulatory light chain of myosin.
• It serves as a motor protein essential for actin-based movement in muscle and nonmuscle cells.
• Unlike some myosin light chains, MYL6B does not bind calcium, indicating a calcium-independent regulatory mechanism.
• Proteomics studies have identified MYL6B in the human proteome, marking it as a reference proteome component.
• The protein features repeat elements, which may contribute to its structural role within the myosin complex.
• Three-dimensional structural data are available for MYL6B, offering insights into its molecular architecture and function.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1–208, representing the full-length human MYL6B; this broad coverage may facilitate recognition of multiple epitopes.
• For western blotting, use reducing conditions and expect a band near 23 kDa; include appropriate loading controls and validate expression in your cell or tissue lysates.
• As a polyclonal antibody, it may exhibit batch-dependent affinity; consider using knockout or siRNA knockdown controls to confirm specificity in your assay system.
• The reported cross-reactivity with mouse and rat suggests utility in rodent models; verify with your experimental samples, as protein sequence homology does not guarantee equivalent detection.
• For ELISA applications, consider using recombinant MYL6B as a standard to calibrate the assay and determine optimal antibody dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a one-stop supply of validated IVD raw materials, encompassing antibody pairs, bulk ancillary reagents, and customized sourcing for challenging targets. For myosin and muscle biology research—exemplified by targets like MYL6B—we help bridge the gap from concept to clinic with reliable, scalable raw-material solutions.