MYL2 Rabbit mAb for WB, IF-P, ELISA - P10916

Core Product Specifications and Parameters

Biological Background: MYL2 Function and Localization

• MYL2 (myosin regulatory light chain 2, ventricular/cardiac muscle isoform) is a contractile protein essential for heart development and function. Related references: PMID:23365102, PMID:32453731
• Phosphorylation of MYL2 enhances cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion, thus fine-tuning myofilament calcium sensitivity.
• During cardiogenesis, MYL2 plays an early role in cardiac contractility by promoting cardiac myofibril assembly.
• The protein localizes to the cytoplasm, specifically within myofibrils at the sarcomere A band.
• MYL2 is highly expressed in type I (slow) muscle fibers, consistent with its role in ventricular and slow skeletal muscle.
• Keywords associated with MYL2 include Calcium, Cardiomyopathy, Metal-binding, Methylation, Motor protein, Muscle protein, Myofibrillar myopathy, Phosphoprotein, and repeat regions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 67–166 of human MYL2 (P10916). When designing similar antibodies, consider this region for epitope targeting.
• For Western blotting, the predicted molecular weight is ~19 kDa; validate detection in relevant cardiac or skeletal muscle lysates.
• In immunofluorescence-paraffin (IF-P) experiments, ensure adequate antigen retrieval and antibody dilution optimization, as staining may reveal sarcomeric A-band patterns.
• ELISA applications may require careful pairing with a suitable detection antibody; cross-reactivity with mouse and rat suggests utility in multi-species studies.
• As the antibody is recombinant and monoclonal, lot-to-lot consistency is expected, but always validate in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs to a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies like this anti-MYL2 clone. Whether you require bulk ancillary reagents or specialized cardiac muscle markers, our sourcing capabilities cover rare and common targets to support your development from concept to clinic. Reach out to explore how we can streamline your cardiac research and assay development with reliable, cost-effective material supply.