MYH6/MYH7 Rabbit PolymAb® for WB, IF-P, IHC-P, ELISA - P13533 / P12883

Core Product Specifications and Parameters

Biological Background: MYH6/MYH7 Function and Localization

• Myosin-6 (MYH6) is the alpha cardiac muscle myosin heavy chain isoform, a major component of the thick filament in cardiac sarcomeres.
• MYH6 functions as a motor protein that hydrolyzes ATP to generate force, driving cardiac muscle contraction.
• It belongs to the myosin heavy chain superfamily, encoded by the MYH6 gene (MYHCA), and is closely related to the beta-cardiac myosin heavy chain (MYH7).
• Localized to the cytoplasm, specifically within myofibrils where it forms the thick filaments of sarcomeres.
• Contains key domains including an actin-binding domain, ATP-binding site, calmodulin-binding region, and a coiled-coil tail for filament assembly; subject to methylation and phosphorylation.
• Mutations in MYH6 are associated with atrial septal defect (ASD3), cardiomyopathy (CMH14, CMD1EE), and other cardiac disorders.
• Classified as a motor protein, muscle protein, and myosin, with annotations for actin-binding, ATP-binding, and disease variant.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide conserved between MYH6 and MYH7; validate isoform specificity using appropriate controls.
• For Western blot, expect a band around 223 kDa; optimize transfer conditions for large proteins (e.g., reduced methanol, extended transfer time).
• IHC-P and IF-P may require optimization of fixation/permeabilization due to the target’s structural role; cross-reactivity with mouse and rat facilitates translational studies.
• The recombinant rabbit monoclonal format (PolymAb®) offers high affinity and batch consistency; confirm performance in your own sample system.
• ELISA applications based on a peptide immunogen may primarily detect linear epitopes; consider this when interpreting results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with integrated access to a comprehensive range of IVD raw materials. For targets like MYH6/MYH7 involved in cardiac muscle biology, we offer validated antibody pairs, recombinant monoclonal antibodies, and ancillary reagents to accelerate your assay development. Whether you need bulk quantities or specialized sourcing for rare analytes, CamelBio streamlines your procurement from concept to clinic.