MYH6/Aα-MHC Rabbit mAb - Q02566

Core Product Specifications and Parameters

Biological Background: Myosin-6 Function and Localization

• Myosin-6, also known as myosin heavy chain 6 (MyHC-alpha), is the alpha cardiac muscle isoform of myosin heavy chain, a key component of the thick filament in sarcomeres.
• This motor protein hydrolyzes ATP to generate the mechanical force required for muscle contraction, interacting with actin filaments in an ATP-dependent manner.
• The protein contains characteristic domains including an actin-binding head, an ATP-binding site, and a long coiled-coil tail that mediates dimerization and filament assembly.
• Subcellular localization is predominantly cytoplasmic, within myofibrils, where it contributes to the structural organization of thick filaments in cardiomyocytes.
• Post-translational modifications such as methylation and phosphorylation have been documented, suggesting regulation of its activity and filament stability.
• As a member of the myosin heavy chain family, it plays an essential role in cardiac muscle physiology and is a reference proteome member, ensuring high-quality functional annotation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 621-720 of mouse MYH6; consider sequence alignment to assess potential cross-reactivity with other myosin isoforms in your sample system.
• Validate the antibody at appropriate dilutions for each application (WB, IF-P, IHC-P, ELISA) using positive and negative controls.
• Given the large protein weight (~224 kDa), use low-percentage SDS-PAGE gels (e.g., 6% resolving gel) and adequate transfer conditions for western blotting.
• For immunofluorescence or immunohistochemistry, include appropriate permeabilization and blocking steps, and verify signal specificity with knockout or knockdown controls if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This MYH6/Aα-MHC antibody supports cardiac muscle research and offers a reliable tool for studying myosin-related contractile mechanisms. Beyond single reagents, we provide tailored sourcing solutions to streamline your development from concept to clinic, covering rare target procurement and large-scale supply. Partner with us to accelerate your project with confidence.