MYH6/α-MHC Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P13533

Core Product Specifications and Parameters

Biological Background: MYH6/α-MHC Function and Localization

• MYH6 (myosin heavy chain 6) encodes the alpha heavy chain subunit of cardiac myosin, a major component of the thick filaments in striated muscle.
• As a motor protein, MYH6 converts chemical energy from ATP hydrolysis into mechanical force, driving muscle contraction in the heart.
• MYH6 is primarily expressed in cardiac muscle and is essential for normal myocardial contractility and heart development.
• The protein localizes to the cytoplasm and incorporates into myofibrils, forming the thick filament together with myosin light chains.
• MYH6 is subject to post-translational modifications such as methylation and phosphorylation, which may regulate its activity and filament assembly.
• Mutations in MYH6 are associated with cardiomyopathies (e.g., familial hypertrophic cardiomyopathy 14) and atrial septal defects, highlighting its clinical significance in cardiac diseases.
• As a reference proteome member, MYH6 is a key target for cardiovascular disease research and diagnostic applications.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a synthetic peptide within amino acids 600-700 of human MYH6. Due to the polyclonal nature, it recognizes multiple epitopes, enhancing detection sensitivity.
• For Western blot (WB), the predicted molecular weight of MYH6 is ~224 kDa; use appropriate gel percentages and transfer conditions for high-molecular-weight proteins. Include cardiac tissue lysates (e.g., human, mouse, rat) as positive controls.
• In immunofluorescence (IF-P), expect staining in the cytoplasm and myofibrils of cardiomyocytes. Optimize permeabilization to ensure antibody penetration into thick filament structures.
• For ELISA, consider coating with the immunogen peptide or recombinant MYH6 protein fragments for quantitative detection. Validate the antibody's reactivity with your specific sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to high-quality IVD raw materials such as optimized antibodies, bulk reagents, and technical services. For cardiac biomarker research and test development, our product portfolio includes validated anti-MYH6/α-MHC antibodies and supporting ancillary materials. Partner with CamelBio to streamline your sourcing from concept to clinic.