MYH6 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P13533

Core Product Specifications and Parameters

Biological Background: MYH6 Function and Localization

• MYH6 encodes myosin-6, also known as cardiac alpha myosin heavy chain (alpha-MHC), a class II myosin motor protein.
• Myosin-6 is the core component of the thick filament in cardiac sarcomeres, converting chemical energy from ATP hydrolysis into mechanical force for muscle contraction.
• The protein localizes to the cytoplasm, specifically to myofibrils and thick filaments within cardiac muscle cells.
• It contains characteristic motor domain features: an N-terminal actin-binding region, an ATP-binding site, and a C-terminal coiled-coil tail responsible for dimerization and filament formation.
• Multiple post-translational modifications, including phosphorylation and methylation, are annotated, suggesting regulatory roles in myosin function and cardiac performance.
• Mutations in MYH6 are associated with human diseases such as atrial septal defect (ASD) and familial hypertrophic cardiomyopathy (CMH), underscoring its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen spans residues 160–260, located within the conserved myosin motor domain, which is highly identical between human and mouse, consistent with the observed cross-reactivity.
• For Western blot, the expected band is ~224 kDa. Ensure prolonged electrophoresis and optimized transfer conditions (e.g., low methanol Towbin buffer) to retain and blot high-molecular-weight proteins efficiently.
• In immunofluorescence on fixed tissue or cells, permeabilize thoroughly and consider anti-fade mounting medium to preserve signal over thick cardiac muscle samples.
• When developing ELISA assays, validate the antibody as capture or detection reagent in the relevant biological matrix, paying attention to potential cross-reactivity with other myosin isoforms present in the sample.
• Non-specific binding can be reduced by using appropriate blocking agents and diluting the antibody in the recommended buffer; empirical optimization is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with integrated access to critical IVD raw materials, including validated monoclonal antibodies like this MYH6 clone. Whether you need matched antibody pairs, bulk reagents, or assistance procuring rare targets in the cardiac biomarker space, our supply network and technical team streamline your product development pipeline from assay concept to clinical application. Contact us to explore tailored sourcing solutions for your cardiac and muscle disease projects.