MYH14 Rabbit mAb for WB, IF-P, ELISA - Q7Z406

Core Product Specifications and Parameters

Biological Background: MYH14 Function and Localization

• Myosin-14, also known as myosin heavy chain 14, non-muscle myosin IIC (NMHC II-C), is encoded by the MYH14 gene and belongs to the myosin superfamily of motor proteins.
• It functions as a cellular myosin involved in cytokinesis, cell shape maintenance, and specialized processes such as secretion and capping.
• Alternative splicing generates multiple isoforms, contributing to functional diversity.
• The protein contains characteristic myosin domains including an ATP-binding motor domain, an actin-binding region, and a coiled-coil tail for dimerization.
• Subcellularly, MYH14 associates with the actin cytoskeleton and is localized to the cytoplasm.
• Post-translational modifications include acetylation and phosphorylation, which regulate its activity and interactions.
• Tissue expression is highest in brain (especially corpus callosum), heart, kidney, liver, lung, small intestine, colon, and skeletal muscle; lower in smooth muscle-rich organs; undetectable in thymus, spleen, placenta, and lymphocytes.
• Mutations in MYH14 are associated with non-syndromic deafness (DFNA4) and peripheral neuropathy, highlighting its role in neurosensory function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide spanning amino acids 600–700, enabling specific detection of MYH14 without cross-reactivity to closely related non-muscle myosins.
• For western blotting, a low-percentage SDS-PAGE gel (e.g., 6%) may be considered to resolve the high molecular weight 228 kDa protein.
• In immunofluorescence (IF-P), validate the antibody in relevant cell lines such as HeLa or neuronal cells that express MYH14, and optimize fixation and permeabilization conditions.
• Cross-reactivity with mouse and rat orthologs allows potential use in rodent model studies; titrate concentrations accordingly.
• For ELISA, confirm coating conditions and detection range using recombinant MYH14 protein or appropriate positive controls.
• Given the protein’s role in cytokinesis, consider synchronizing cell cultures when studying expression dynamics.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. In the area of cytoskeletal and motor protein research, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare targets like MYH14, CamelBio’s supply chain capabilities ensure reliable access to high-quality raw materials, enabling seamless integration into diagnostic assay development.