MYF6 Rabbit pAb for WB and ELISA - P23409

Core Product Specifications and Parameters

Biological Background: MYF6 Function and Localization

• MYF6 (Myogenic factor 6) is a muscle-specific transcription factor known as Class C basic helix-loop-helix protein 4 (bHLHc4) or Muscle-specific regulatory factor 4 (MRF4).
• It functions as a myogenic factor, playing a critical role in muscle differentiation by inducing fibroblast-to-myoblast conversion.
• MYF6 acts as a probable sequence-specific DNA-binding protein, regulating gene expression essential for skeletal muscle development.
• Subcellular localization is nuclear, consistent with its role as a transcription factor.
• Expression is restricted to skeletal muscle tissue, highlighting its tissue-specific regulatory function.
• The protein belongs to the basic helix-loop-helix family and is classified under developmental proteins with roles in differentiation and myogenesis.
• The canonical isoform mass is approximately 27 kDa (calculated 26,953 Da), matching the detected band in Western blot applications.

Experimental Guidance and Technical Tips

• The immunogen (aa 80-180) spans the bHLH domain, ensuring reactivity against functionally critical regions; verify specificity using appropriate controls.
• For Western blot, a band around 27 kDa is expected in human, mouse, or rat skeletal muscle lysates; optimize detection conditions.
• This antibody can be applied in ELISA; consider pairing with a suitable MYF6 capture antibody for sandwich ELISA development.
• Cross-reactivity with mouse and rat allows translation between human clinical samples and rodent models; however, sequence alignment is advised.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material partner, CamelBio supports your skeletal muscle research and diagnostic development by supplying this MYF6 polyclonal antibody along with a comprehensive range of validated antibody pairs, recombinant proteins, and bulk ancillary reagents. Whether you need optimized monoclonal antibodies, rare target sourcing, or technical consulting, our integrated supply chain streamlines your transition from early-stage assay design to clinical application.