MYF5 Rabbit pAb for WB - P13349

Core Product Specifications and Parameters

Biological Background: MYF5 Function and Localization

• Protein identity: Myogenic factor 5 (MYF5), also known as Class C basic helix-loop-helix protein 2 (bHLHc2), is a key member of the myogenic regulatory factor family.
• Core function: MYF5 acts as a transcriptional activator that promotes the expression of muscle-specific genes and plays a pivotal role in muscle differentiation. Related references: PMID:29887215
• It cooperates with other myogenic factors, such as MYOG and MYOD1, to co-occupy the core promoter regions of muscle-specific genes during myogenesis.
• MYF5 can induce fibroblasts to differentiate into myoblasts, highlighting its role in cell fate determination.
• Subcellular localization: MYF5 is predominantly found in the nucleus, consistent with its function as a DNA-binding transcription factor.
• Associated biological processes include myogenesis, developmental protein activity, and transcriptional regulation.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 143–220 of human MYF5. Consider verifying antibody reactivity with your target isoform, especially in light of potential post-translational modifications or splice variants.
• For Western blotting, a band around 28 kDa is expected. Optimal dilution and experimental conditions should be empirically determined using appropriate positive and negative control lysates.
• The antibody has been reported to cross-react with mouse MYF5, making it suitable for studies in murine models. Validate cross-reactivity in your specific sample system.
• For ELISA, the antibody can be assessed in direct or sandwich formats; pairing with other MYF5 antibodies may be explored for assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material sourcing partner, supporting diagnostic manufacturers, laboratories, and research institutions from concept to clinic. For your myogenesis and developmental biology projects, we offer this anti-MYF5 rabbit polyclonal antibody alongside a broad portfolio of validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing services. Our one-stop supply chain ensures consistent quality and timely delivery for your assay development needs.