MYB Rabbit pAb for WB, ELISA - P10242

Core Product Specifications and Parameters

Biological Background: MYB Function and Localization

• Transcriptional activator Myb (proto-oncogene c-Myb) is a DNA-binding protein that specifically recognizes the sequence 5'-YAAC[GT]G-3'.
• Plays a critical role in the control of proliferation and differentiation of hematopoietic progenitor cells.
• Localizes to the nucleus, consistent with its function as a transcription factor.
• Undergoes alternative splicing, generating multiple isoforms.
• Subject to various post-translational modifications including acetylation, phosphorylation, and ubiquitin conjugation (isopeptide bond), which regulate its activity and stability.
• Contains DNA-binding repeats characteristic of the Myb family.
• Classified as a proto-oncogene; aberrant expression or mutations are implicated in oncogenesis.

Experimental Guidance and Technical Tips

• When using this polyclonal antibody for Western blot, consider validating in human cell lines known to express MYB (e.g., hematopoietic cells) and use appropriate loading controls. The immunogen sequence (aa 380-640) may detect multiple isoforms due to alternative splicing.
• For ELISA, optimize coating antigen concentration and detection system to achieve desired sensitivity and specificity.
• Based on the predicted protein weight of 72 kDa, adjust gel percentage and transfer conditions accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, technical services, and consulting to support diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This MYB antibody serves as a reliable tool for investigating transcriptional regulation and hematopoietic malignancies. Explore our catalog for validated antibody pairs, bulk ancillary reagents, and customized sourcing solutions for rare targets.