mTOR Rabbit pAb - P42345

Core Product Specifications and Parameters

Biological Background: mTOR Function and Localization

• mTOR (Mechanistic Target of Rapamycin) is a serine/threonine protein kinase that serves as a central regulator of cellular metabolism, growth, and survival, integrating signals from hormones, growth factors, nutrients, energy, and stress. Related references: PMID:12087098 PMID:12150925 PMID:12150926
• It functions within two distinct complexes: mTORC1 and mTORC2. mTORC1 is nutrient-sensitive and promotes anabolic processes like protein, lipid, and nucleotide synthesis, while mTORC2 is nutrient-insensitive and regulates proliferation, survival, and cytoskeletal organization by activating AGC kinases such as AKT, PKC, and SGK1. Related references: PMID:15268862 PMID:15467718 PMID:18497260 PMID:21376236 PMID:24670654
• mTORC1 directly phosphorylates key translational regulators including EIF4EBP1 (4E-BP1) and RPS6KB1 (S6K), thereby enhancing mRNA translation and ribosome biogenesis. Related references: PMID:12087098 PMID:12150925 PMID:12150926 PMID:24403073 PMID:29236692
• mTORC1 also regulates lipid synthesis via SREBF1 and suppresses autophagy by phosphorylating ULK1 and other autophagy-related proteins. Related references: PMID:23426360 PMID:32561715 PMID:20537536
• mTORC2-mediated phosphorylation of AKT at Ser473 is critical for full AKT activation, influencing cell survival, metabolism, and migration. Related references: PMID:15268862 PMID:15467718 PMID:21376236 PMID:24670654
• Subcellularly, mTOR dynamically localizes to lysosomes, endoplasmic reticulum, Golgi apparatus, mitochondria, nucleus, and cytoplasmic vesicles in response to nutrient and growth factor cues.
• mTOR is widely expressed across tissues, with the highest levels observed in the testis.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to the N-terminal region (amino acids 1-300) of human mTOR. This sequence is conserved across human, mouse, and rat, consistent with the reported cross-reactivity.
• For Western blot (WB), expect a band at approximately 289 kDa. Use appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In immunoprecipitation (IP), the antibody may pull down mTOR and its associated complex components; validate under native conditions.
• For immunofluorescence (IF/ICC), staining may appear in cytoplasmic, lysosomal, and membrane compartments depending on cell type and culture conditions. Include appropriate controls.
• Optimal dilutions and conditions should be determined empirically for each application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting, covering every stage from concept to clinic. This mTOR polyclonal antibody supports research in cell signaling, cancer biology, and metabolic disorders. Complement your assay development with our validated antibody pairs, optimized bulk reagents, and rare-target sourcing capabilities.