MTIF2 Rabbit pAb - P46199

Core Product Specifications and Parameters

Biological Background: MTIF2 Function and Localization

• The mitochondrial translation initiation factor IF-2, encoded by the MTIF2 gene, is a critical GTPase for organellar protein biosynthesis.
• Its primary function is to deliver formylmethionyl-tRNA (fMet-tRNA) to the small 30S ribosomal subunit, positioning the initiator tRNA at the start codon.
• MTIF2 catalyzes GTP hydrolysis during joining of the 50S subunit to form the 70S initiation complex, a process that drives translation initiation forward.
• The protein is strictly localized to the mitochondrial matrix, where mitochondrial DNA-encoded mRNAs are translated.
• Expression of MTIF2 is widespread, with the highest levels in skeletal muscle, consistent with the high demand for mitochondrial protein synthesis in muscle tissue.
• As a phosphoprotein and a GTP-binding protein, MTIF2 may be subject to regulatory phosphorylation and nucleotide-dependent conformational changes, influencing mitochondrial translation activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal domain (aa 428-727), which may be critical for recognizing the native mitochondrial form of MTIF2.
• For Western blotting (WB), a band near 81 kDa is expected; consider using mitochondrial-enriched lysates to enhance signal.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), target localization should be mitochondrial; validate co-staining with mitochondrial markers.
• The antibody detects MTIF2 in human, mouse, and rat samples, but cross-reactivity with other species should be verified by sequence comparison of the immunogen region.
• ELISA applications may be used for quantification; optimize coating antigen and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to validated IVD raw materials including this polyclonal antibody targeting mitochondrial translation factor MTIF2. From monoclonal antibodies and optimized ELISA pairs to bulk ancillary reagents, we support diagnostic manufacturers in mitochondrial and metabolic disease research. Our expert team also assists with rare target sourcing and assay development guidance.