MTHFR Rabbit Polyclonal Antibody for WB, ELISA - P42898

Core Product Specifications and Parameters

Biological Background: MTHFR Function and Localization

• MTHFR catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a methyl donor for homocysteine remethylation to methionine (PubMed:29891918). Related references: PMID:29891918
• The enzyme acts as a key regulatory connection between the folate and methionine cycles.
• MTHFR is an allosteric enzyme that requires FAD and NADPH as cofactors and is regulated by S-adenosylmethionine.
• Alternative splicing generates multiple isoforms, contributing to functional diversity.
• MTHFR undergoes phosphorylation, a post-translational modification that may modulate enzyme activity.
• Genetic variants in MTHFR are associated with disorders, including schizophrenia and cardiovascular conditions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–230); consider that the antibody may recognize both full-length and truncated isoforms.
• For Western blot analysis, a band at approximately 75 kDa is anticipated; validate the signal using appropriate positive and negative controls.
• Cross-reactivity has been observed with rat samples; test the antibody in the relevant species system before routine use.
• For ELISA applications, optimize coating and detection conditions using known antigen standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with comprehensive IVD raw material solutions, covering every stage from concept to clinic. This polyclonal antibody targeting MTHFR supports investigations into folate and methionine metabolism, and we can further assist with validated antibody pairs, bulk reagent supply, and sourcing of rare-target raw materials to accelerate your assay development.