MTAP Rabbit Monoclonal Antibody for WB, ELISA - Q13126

Core Product Specifications and Parameters

Biological Background: MTAP Function and Localization

• MTAP (S-methyl-5'-thioadenosine phosphorylase) catalyzes the phosphorolytic cleavage of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate, initiating the methionine salvage pathway.
  Related references: PMID:3091600, PMID:8687427, PMID:10404592

• It plays a critical role in polyamine biosynthesis by recycling MTA, a major by-product of this process.
  Related references: PMID:3091600, PMID:8687427, PMID:10404592

• The enzyme exhibits broad substrate specificity, with a preference for 6-aminopurine nucleosides.
  Related references: PMID:10404592

• MTAP is localized in both the cytoplasm and the nucleus.

• It is ubiquitously expressed across human tissues.

• Post-translational modifications such as acetylation and alternative splicing generate multiple isoforms.

• The protein is classified under the purine salvage pathway and has been identified by proteomics as a reference proteome member.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length N-terminal region (amino acids 1–283) of human MTAP, suggesting broad epitope coverage. For Western blot, a band at ~31 kDa is expected. Optimize antibody concentration and detection conditions for your specific sample system.
• This antibody cross-reacts with mouse and rat MTAP, making it suitable for comparative studies in rodent models. Confirm cross-reactivity in your assays.
• MTAP expression is often lost in certain cancers, so consider using appropriate positive and negative controls (e.g., MTAP-proficient vs. deficient cell lines) when assessing specificity.
• For ELISA, pairing with another clone or a polyclonal detection antibody may improve sensitivity; perform titration experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This MTAP antibody supports research in polyamine metabolism and cancer diagnostics. For your large-scale or custom reagent needs, our one-stop sourcing services can accelerate your project from concept to clinic.