MT-ND1 Rabbit Polyclonal Antibody for WB and ELISA - P03888

Core Product Specifications and Parameters

Biological Background: MT-ND1 Function and Localization

• MT-ND1 encodes NADH-ubiquinone oxidoreductase chain 1, a core subunit of mitochondrial respiratory chain Complex I.
• It is essential for the catalytic activity and proper assembly of Complex I, mediating electron transfer from NADH to ubiquinone.
• The protein localizes to the mitochondrial inner membrane as a multi-pass transmembrane protein, anchoring Complex I within the membrane.
• Complex I is the primary entry point for electrons into the respiratory chain, critical for cellular ATP production and metabolism.
• UniProtKB keywords include Electron transport, Respiratory chain, Translocase, and Ubiquinone, highlighting its central role in oxidative phosphorylation.
• The mouse MT-ND1 protein shares high conservation with human MT-ND1, enabling cross-species recognition.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N-terminal region (amino acids 1–100) of mouse MT-ND1, which may allow detection of both denatured and native forms.
• For Western blot (WB), consider using mitochondrial-enriched lysates from human or mouse tissues to achieve optimal signal, as MT-ND1 is a mitochondrial membrane protein.
• In ELISA, the antibody may be suitable for direct or capture formats; validate performance with recombinant MT-ND1 or cell-based assays in the relevant sample system.
• Given the cross-reactivity with human and mouse, this antibody can be used for comparative studies, but always include appropriate species-specific controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials—from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents—supporting every stage of assay development. For mitochondrial research targets like MT-ND1, our portfolio streamlines procurement of high-quality reagents. We also specialize in rare target raw-material sourcing, acting as a reliable bridge to bring your concepts from bench to clinic.