MT-ND1 Rabbit Polyclonal Antibody (Anti-NADH Dehydrogenase Subunit 1) for WB, IHC-P, IF/ICC, ELISA - P03886

Core Product Specifications and Parameters

Biological Background: MT-ND1 Function and Localization

• MT-ND1 encodes NADH-ubiquinone oxidoreductase chain 1, also known as NADH dehydrogenase subunit 1, a core component of mitochondrial Complex I.
• It is essential for the catalytic activity and assembly of Complex I, which catalyzes electron transfer from NADH to ubiquinone in the respiratory chain. Related references: PMID:1959619 PMID:26929434
• MT-ND1 is located in the mitochondrial inner membrane, consistent with its role in oxidative phosphorylation.
• The protein is a multi-pass transmembrane subunit that contributes to the structural integrity of Complex I.
• Mutations in MT-ND1 are associated with mitochondrial diseases, including Leber hereditary optic neuropathy (LHON), MELAS syndrome, and Alzheimer disease, highlighting its clinical importance.
• The predicted molecular weight is approximately 35.7 kDa, consistent with the observed band around 36 kDa in western blotting.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human MT-ND1. Consider this sequence specificity when interpreting cross-reactivity data.
• For western blotting (WB), a band at approximately 36 kDa is expected. Validate detection in mitochondrial-enriched fractions or whole cell lysates from human, mouse, or rat samples.
• When performing immunohistochemistry (IHC-P) or immunofluorescence (IF/ICC), mitochondrial staining patterns should be confirmed; consider co-staining with a mitochondrial marker.
• Due to the polyclonal nature of the antibody, lot-to-lot consistency should be verified for critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting your projects from conceptualization to clinical application. This anti-MT-ND1 antibody is tailored for mitochondrial research and complex I studies. Alongside this product, we offer a full portfolio of validated antibody pairs, recombinant proteins, and ancillary reagents for IVD development and bulk supply.