MST4 Rabbit Monoclonal Antibody for WB and ELISA - Q9P289

Core Product Specifications and Parameters

Biological Background: MST4 Function and Localization

• MST4 (STK26), also designated as MASK, MST-4, or serine/threonine-protein kinase 26, is a serine/threonine kinase involved in cell growth modulation and apoptosis regulation. Related references: PMID:11641781, PMID:17360971
• In complex with STK24, MST4 negatively regulates Golgi reorientation during polarized cell migration following RHO activation. Related references: PMID:27807006
• The kinase directly phosphorylates ATG4B at serine-383, thereby increasing autophagic flux. Related references: PMID:29232556
• MST4 is a component of striatin-interacting phosphatase and kinase (STRIPAK) complexes, which regulate critical signaling pathways including Hippo, MAPK, nuclear receptor, and cytoskeleton remodeling. Related references: PMID:18782753
• Subcellularly, MST4 localizes to the cytoplasm and Golgi apparatus, consistent with its role in Golgi dynamics.
• The protein features an ATP-binding domain and requires magnesium as a cofactor, classifying it as a serine/threonine-protein kinase.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 317-416) of human MST4, which is conserved across human, mouse, and rat; consider this region's location when interpreting domain-specific functional assays.
• For Western blot, the predicted molecular weight is approximately 47 kDa (UniProt: 46.5 kDa); use a positive control such as human or mouse cell lysates known to express MST4.
• In ELISA, titrate the antibody and optimize coating conditions in your specific assay format (direct, indirect, or sandwich).
• Validate cross-reactivity in your target species by comparing endogenous band patterns or using recombinant protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, technical services, and consulting. Our MST4 (STK26) monoclonal antibody supports immunoassay development for studies on cell growth, apoptosis, and autophagy. Contact us to meet your sourcing needs for validated antibody pairs, optimized antibodies, bulk ancillary reagents, or rare-target procurement.