MSR1 Rabbit mAb for WB, ELISA - P21757

Core Product Specifications and Parameters

Biological Background: MSR1 Function and Localization

• MSR1 encodes macrophage scavenger receptor types I and II, also known as CD204 or SCARA1, which belong to the class A scavenger receptor family and undergo alternative splicing to generate multiple isoforms (Isoform I, II, and III).
• The receptor functions as a membrane glycoprotein that binds, endocytoses, and phagocytoses a broad range of ligands, including modified low-density lipoproteins such as acetylated LDL and oxidized LDL, thereby promoting cholesterol uptake and contributing to foam cell formation during atherogenesis.
  Related references:
  PMID:2251254 PMID:34252459
• MSR1 plays a critical role in innate immune defense by recognizing pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs), including bacterial cell wall components and apoptotic debris, facilitating macrophage clearance activity.
  Related references:
  PMID:10722588
• Upon stimulation by CpG DNA, MSR1 mediates activation of LYN kinase, which phosphorylates SYK and promotes its recruitment to endosomal TLR9, thereby enhancing downstream TLR9 signaling.
  Related references:
  PMID:29057947 PMID:40980882
• The receptor is localized to the cell membrane and exhibits tissue-specific expression: isoforms I, II, and III are found in monocyte-derived macrophages, while isoforms I and II are additionally expressed in the liver, placenta, and brain.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, contributing to its structural stability and ligand-binding properties.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–100 of human MSR1. For Western blotting, consider using reducing conditions and validate the observed band against the expected molecular weight (approximately 50 kDa) in lysates from human, mouse, or rat macrophage cell lines or tissues known to express MSR1.
• For ELISA applications, the peptide-based immunogen may preferentially detect linear epitopes; it is advisable to validate the assay with recombinant MSR1 protein or relevant biological fluids to confirm sensitivity and specificity.
• Cross-reactivity is predicted with mouse and rat MSR1 based on sequence homology; however, experimental confirmation is recommended for each species in the intended sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as a trusted one-stop IVD raw-material partner, streamlining your access to high-quality antibodies and ancillary reagents from discovery to diagnostic development. The MSR1 monoclonal antibody serves researchers investigating macrophage biology, atherosclerosis, and innate immunity, and CamelBio’s extensive supply network ensures consistent availability of validated antibody pairs, bulk monoclonal/polyclonal antibodies, and customized sourcing for rare or specialized targets. Partner with CamelBio to accelerate your assay development with expert technical support and reliable, scalable material supply.