MRPS36 Rabbit pAb for WB, IHC-P, ELISA - P82909

Core Product Specifications and Parameters

Biological Background: Alpha-ketoglutarate dehydrogenase component 4 (MRPS36) Function and Localization

• MRPS36, also known as alpha-ketoglutarate dehydrogenase component 4 or KGD4, acts as a molecular adapter required for the assembly of a stable 2-oxoglutarate dehydrogenase enzyme complex (OGDHC).
• It specifically facilitates the recruitment of the E3 subunit (dihydrolipoamide dehydrogenase) to the E2 subunit (dihydrolipoamide succinyltransferase) within OGDHC, ensuring proper complex formation.
• The protein is localized exclusively to the mitochondrion, where it participates in the tricarboxylic acid (TCA) cycle by supporting the conversion of alpha-ketoglutarate to succinyl-CoA.
• Post-translational modifications include acetylation and phosphorylation, as identified in high-throughput proteomics studies.
• Although historically designated as a mitochondrial ribosomal protein (MRPS36), current functional annotations confirm its primary role is not in the ribosome but as an essential structural component of the OGDHC.
• The gene KGD4/MRPS36 is conserved across species, and the protein is a single-pass membrane protein with a molecular weight of approximately 11 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal portion (amino acids 1–103) of human MRPS36, which may target a conserved region; validate reactivity in rat samples if needed using appropriate controls.
• For Western blot, detect the band near 11 kDa using standard SDS-PAGE and loading controls; consider using mitochondrial-enriched fractions to enhance signal specificity.
• In IHC-P, antigen retrieval may optimize epitope exposure; test with known positive mitochondrial-rich tissues (e.g., liver, heart).
• ELISA applications may use the antibody as a capture or detection reagent; confirm specificity with recombinant MRPS36 protein or lysates from cells with validated expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw-material procurement by providing access to a broad portfolio of validated antibodies, including this MRPS36 polyclonal antibody for mitochondrial metabolism studies. Whether you require customized antibody pairs, bulk monoclonal or polyclonal antibodies, or ancillary reagents, our team can coordinate sourcing and technical support from discovery to commercial readiness. For researchers delving into TCA cycle enzymology or mitochondrial dysfunction, CamelBio ensures a reliable supply of high-quality raw materials tailored to your diagnostic development needs.