MRPS27 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q92552

Core Product Specifications and Parameters

Biological Background: MRPS27 Function and Localization

• MRPS27 encodes the mitochondrial ribosomal protein mS27 (also known as MRP-S27 or S27mt), a component of the small 28S subunit of the mitochondrial ribosome.
• It functions as an RNA-binding protein that interacts with the 12S mitochondrial rRNA and tRNA(Glu) to facilitate mitochondrial protein synthesis, specifically stimulating translation of electron transport chain subunit mRNAs (PubMed:22841715). Related references: PMID:22841715
• MRPS27 has been shown to positively regulate cell proliferation and promote tumor cell growth, with mechanistic studies linking it to enhanced mitochondrial translation in cancer cells (PubMed:28714366). Related references: PMID:28714366
• Subcellular localization studies indicate MRPS27 is present in both mitochondria and the cytoplasm, reflecting its ribosome-associated and potential extra-ribosomal functions.
• The protein is expressed ubiquitously across human tissues, but its overexpression is a hallmark of hepatocellular carcinoma and possibly other malignancies (PubMed:22841715; PubMed:28714366). Related references: PMID:22841715 | PMID:28714366
• MRPS27 is classified as a ribosomal protein with RNA-binding properties, featuring a transit peptide for mitochondrial import and containing coiled-coil regions; it is implicated in translation regulation and has known alternative splicing variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 255-414) of human MRPS27, which is predicted to be accessible in the native protein and suitable for detecting both endogenous and overexpressed MRPS27 by WB.
• For Western blot, a band at approximately 48 kDa is expected; consider optimizing loading controls and antibody dilution for your specific sample lysate.
• In IF/ICC, pre-validation of fixation and permeabilization conditions is recommended to preserve mitochondrial morphology and antigenicity.
• ELISA applications may require careful titration of coating antigen and antibody concentration; the antibody’s reactivity with mouse and rat orthologs suggests cross-species utility in rodent models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials. For mitochondrial biology and cancer research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies such as this anti-MRPS27 antibody, and bulk ancillary reagents to streamline your assay development. Our one-stop sourcing model covers everything from concept to clinic, ensuring reliable access to rare-target raw materials and technical consulting.