MRPS16 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y3D3

Core Product Specifications and Parameters

Biological Background: MRPS16 Function and Localization

• MRPS16 encodes the small ribosomal subunit protein bS16m, a component of the mitochondrial ribosome (mitoribosome) responsible for synthesizing 13 essential proteins of the oxidative phosphorylation system.
• Also known as 28S ribosomal protein S16, mitochondrial (MRP-S16), it is part of the small 28S subunit, contributing to the structural integrity and function of the mitoribosome.
• The protein is nuclear-encoded, synthesized with a mitochondrial targeting sequence (transit peptide) that directs its import into the mitochondrial matrix.
• Alternative splicing of the MRPS16 gene transcript generates multiple isoforms, which may have tissue-specific expression or regulatory roles.
• MRPS16 is a phosphoprotein, and post-translational modifications such as phosphorylation could modulate its assembly, activity, or interaction with other mitochondrial proteins.
• Mutations in this gene are linked to combined oxidative phosphorylation deficiency 2 (COXPD2), a primary mitochondrial disease characterized by lactic acidosis, hypotonia, and poor growth.
• The three-dimensional structure of MRPS16 within the mitoribosome has been elucidated, providing insights into the unique features of mitochondrial translation machinery.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 137 amino acids of human MRPS16, which includes the transit peptide and portions of the mature protein. The polyclonal nature may result in recognition of multiple epitopes, potentially detecting both precursor and mature forms.
• For Western blotting, expect a band at approximately 15 kDa. To improve signal, consider using mitochondrial-enriched protein extracts, as MRPS16 is localized to mitochondria.
• In immunofluorescence/immunocytochemistry, verify mitochondrial localization by co-staining with a mitochondrial marker (e.g., MitoTracker or anti-TOM20) and use appropriate fixation/permeabilization protocols.
• The antibody shows cross-reactivity with mouse and rat MRPS16. Validate its specificity in the relevant species and tissue type before quantitative assays.
• For ELISA, optimize coating antigen concentration and antibody dilution. The polyclonal antibody may be used as a capture or detection reagent, but pairing with a monoclonal antibody might be necessary for high-sensitivity sandwich ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and biotech companies with one-stop access to high-quality IVD raw materials. Our portfolio includes validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert sourcing for rare targets. For mitochondrial research and translational assays, this MRPS16 antibody is part of our comprehensive immunoassay solutions, and we offer additional reagents to support your entire development workflow, from concept to clinic.