MRPL34 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BQ48

Core Product Specifications and Parameters

Biological Background: MRPL34 Function and Localization

• MRPL34 encodes the large ribosomal subunit protein bL34m, also designated 39S ribosomal protein L34, mitochondrial (L34mt or MRP-L34).
• It is a component of the mitochondrial ribosome, specifically the large subunit, and thereby participates in mitochondrial protein synthesis.
• The protein is targeted to the mitochondrion and contains a transit peptide for mitochondrial import.
• It is classified as a ribonucleoprotein and a ribosomal protein, reflecting its structural role in the ribosomal complex.
• Post-translational modifications include phosphorylation, and its three-dimensional structure has been determined.
• The mature protein has a molecular weight of approximately 10 kDa.

Experimental Guidance and Technical Tips

• For Western blot, an expected band is observed near 10 kDa; consider using knockdown or knockout controls to confirm specificity.
• In immunofluorescence/immunocytochemistry (IF/ICC), co-staining with a mitochondrial marker is recommended to verify localization.
• For ELISA applications, optimize coating antigen concentration and antibody dilution using a checkerboard titration.
• Given the reported cross-reactivity with mouse and rat, validate performance in these species by testing appropriate tissue or cell lysates, as sequence homology may vary.
• The polyclonal nature of this antibody may provide signal amplification but could also increase background; include proper negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers and research institutions, offering one-stop access to IVD raw materials. This MRPL34 antibody supports studies in mitochondrial biology and protein synthesis, and fits into broader panels for metabolic or mitochondrial disorder research. Beyond individual antibodies, CamelBio supplies validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to streamline your assay development from concept to clinic.