MRPL20 Rabbit Monoclonal Antibody (KD Validated) for WB, IHC-P, ELISA - Q9BYC9

Core Product Specifications and Parameters

Biological Background: MRPL20 (Large ribosomal subunit protein bL20m) Function and Localization

• MRPL20 encodes the human 39S ribosomal protein L20 (L20mt), a component of the large subunit of the mitochondrial ribosome.
• Alternate names include bL20m and MRP-L20, reflecting its role as a mitochondrial ribosomal protein.
• The protein is specifically localized to the mitochondrial compartment, where it participates in the assembly and function of the mitoribosome.
• It is synthesized with a mitochondrial transit peptide that directs its import into the organelle.
• Alternative splicing generates multiple transcript variants, potentially contributing to regulatory diversity.
• As a ribosomal protein, MRPL20 is a ribonucleoprotein involved in mitochondrial protein biosynthesis and maintenance of mitochondrial function.
• The protein’s calculated molecular weight is approximately 17 kDa (UniProt: 17443 Da), consistent with the observed band on Western blot.

Experimental Guidance and Technical Tips

• This monoclonal antibody has been validated in knockdown (KD) experiments, confirming its specificity for MRPL20.
• For Western blot, a band around 17 kDa is expected in mitochondria-enriched lysates; human, mouse, and rat samples are compatible.
• In IHC-P, consider antigen retrieval methods (e.g., citrate buffer, heat-induced) to expose epitopes in mitochondrial proteins.
• The immunogen corresponds to a region (aa 48–147) conserved across tested species, supporting cross-reactivity in rat and mouse.
• For ELISA, pair with an appropriate detection antibody and validate in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with high-quality IVD raw materials, including validated antibodies for mitochondrial targets like MRPL20. We offer this rabbit monoclonal antibody in bulk quantities, along with complementary reagents such as antigen proteins and ELISA kits, to streamline your assay development pipeline. For specialized needs, our team can source rare targets or develop custom monoclonals, ensuring a seamless path from concept to clinic.