MRPL1 Rabbit pAb - Q9BYD6

Core Product Specifications and Parameters

Biological Background: Large ribosomal subunit protein uL1m Function and Localization

• MRPL1 encodes the mitochondrial ribosomal protein L1, also known as 39S ribosomal protein L1, mitochondrial (L1mt, MRP-L1), which is a component of the large subunit of the mitochondrial ribosome.
• The protein is essential for mitochondrial translation, facilitating the synthesis of the 13 mitochondrial DNA-encoded subunits of the oxidative phosphorylation complexes.
• As a ribosomal protein, MRPL1 binds directly to the 16S rRNA within the 39S large subunit and is involved in ribosome assembly and stability.
• Localized exclusively to the mitochondrial matrix, the protein contains an N-terminal transit peptide that directs its import into the organelle.
• Post-translational modifications or processing of the transit peptide may regulate its integration into the ribosome, though specific modifications remain to be fully characterized.
• Structurally, MRPL1 belongs to the universal ribosomal protein uL1 family, sharing homology with bacterial and eukaryotic cytoplasmic L1 proteins.
• Keywords associated with this protein include ribonucleoprotein, transit peptide, and mitochondrion, reflecting its role and localization.

Experimental Guidance and Technical Tips

• For Western blot, a band corresponding to the full-length MRPL1 is expected around 37 kDa (calculated molecular weight ~36.9 kDa). Consider using a mitochondrial-enriched lysate from human or rat cells to improve detection.
• Validate the antibody in the relevant sample system (e.g., HeLa or HEK293 cells) to confirm specificity, as cross-reactivity with other ribosomal proteins is theoretically low but should be assessed.
• In ELISA, the immunogen-derived recombinant protein covering amino acids 51–325 may serve as a positive control, and coating conditions should be optimized empirically.
• Because MRPL1 is a mitochondrial protein, subcellular fractionation can help enrich the target and reduce background from abundant cytosolic ribosomal proteins.

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