MR1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q95460

Core Product Specifications and Parameters

Biological Background: MR1 Function and Localization

• MR1 (major histocompatibility complex class I-related protein 1), also known as class I histocompatibility antigen-like protein, is an MHC I-like molecule encoded by the MR1 gene.
• Functions as an antigen-presenting molecule that displays microbial riboflavin-derived pyrimidine metabolites to semi-invariant TCRs on MAIT cells, triggering innate-like immune responses at mucosal barriers and promoting pathogen clearance. Related references: PMID:19416870, PMID:23457030, PMID:22692454, PMID:20581831, PMID:24101382, PMID:24695216, PMID:23846752
• Presents signature antigens such as 5-OE-RU and 5-OP-RU generated from riboflavin pathway intermediates, and can also capture non-microbial metabolites like tumor-associated pyridoxal or oxidative stress adducts (e.g., M3Ade). Related references: PMID:24695216, PMID:32958637, PMID:32709702, PMID:31959982, PMID:39589872, PMID:39701104
• Allelic variants (MR101 and MR104) modulate ligand repertoires; MR101 preferentially presents oxidative carbonyl adducts, while MR104 presents tumor-enriched pyridoxal and can act as an alloantigen. Related references: PMID:39589872, PMID:39701104
• Localizes to multiple cellular membranes, including the cell surface, endoplasmic reticulum, Golgi apparatus, and endosomes; a secreted form is also detected, consistent with immune surveillance and antigen sampling functions.
• Ubiquitously expressed at low levels; enriched in thymocytes, plasma B cells in the gut lamina propria, and bronchial epithelial cells, reflecting its role in mucosal and systemic immunity. Related references: PMID:7624800, PMID:9780177, PMID:27043408, PMID:19760593, PMID:22692454
• Contains an immunoglobulin-like domain, disulfide bonds, and N-linked glycosylation sites, characteristic of MHC class I molecules and critical for protein folding and ligand binding.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 23–291, covering the majority of the MR1 extracellular domain; expect broad epitope recognition typical of polyclonal antibodies.
• For Western blot (WB), reducing conditions are recommended; a band near 39 kDa should be observed in human, mouse, or rat samples, though post-translational modifications may cause slight shifts.
• For immunofluorescence/immunocytochemistry (IF/ICC), consider optimization of fixation and permeabilization, as MR1 cycles between intracellular membranes and the cell surface.
• ELISA applications may benefit from pairing this antibody with a suitable detection reagent; validate performance in your specific assay format.
• Cross-reactivity with mouse and rat MR1 is indicated, but sequence alignment of the immunogen region with your species of interest is advised to confirm potential recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a reliable one-stop supply of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. For targets like MR1, which bridge innate immunity and cancer immune surveillance, CamelBio streamlines procurement from bulk antibody orders to rare-target reagent sourcing, supporting your workflow from concept to clinical application.