MPHOSPH6 Rabbit Polyclonal Antibody for WB, ELISA - Q99547

Core Product Specifications and Parameters

Biological Background: MPHOSPH6 Function and Localization

• MPHOSPH6 (also known as MPP6, MPP, MPP-6) is an RNA-binding protein that plays a role in rRNA processing.
• It associates with the RNA exosome complex and is involved in the 3'-end processing of 7S pre-rRNA to mature 5.8S rRNA.
• MPHOSPH6 helps recruit the RNA exosome complex to pre-rRNA, a process that may involve the cofactor C1D.
• The protein localizes predominantly to the nucleolus and nucleus, but can also be found in the cytoplasm.
• Post-translational modifications include phosphorylation (as implied by its name, M-phase phosphoprotein 6) and ubiquitination (isopeptide bond formation).
• Structural studies indicate its involvement in RNA binding and exosome complex assembly.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering the N-terminal 160 amino acids of human MPHOSPH6, which is well-conserved across human, mouse, and rat, supporting cross-reactivity in these species.
• For Western blot analysis, validate the antibody using lysates from human, mouse, or rat cells expressing MPHOSPH6. Consider optimizing antibody dilution and exposure times based on your specific sample type and detection system.
• In ELISA applications, pair this antibody with an appropriate recombinant MPHOSPH6 protein or peptide as the coating antigen. Test a range of antibody concentrations to achieve optimal signal-to-noise ratio.
• This polyclonal antibody may recognize multiple epitopes, potentially enhancing detection sensitivity but also warranting careful validation against potential nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers streamlined access to premium IVD raw materials, including this MPHOSPH6 polyclonal antibody for RNA processing studies. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal reagents, and bulk ancillary materials, supporting your entire development pipeline from target discovery to clinical application. We help source even rare targets, ensuring you have the critical reagents needed for every stage of diagnostic development.