MPC1 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q9Y5U8

Core Product Specifications and Parameters

Biological Background: MPC1 Function and Localization

• MPC1, also known as brain protein 44-like protein, is a component of the mitochondrial pyruvate carrier complex that transports pyruvate across the inner mitochondrial membrane.
• It mediates the uptake of pyruvate into mitochondria to maintain the balance between glycolysis and oxidative phosphorylation. Related references: PMID:22628558, PMID:26253029, PMID:27317664
• The protein plays an essential role in cellular metabolism, supporting energy production and biosynthesis. Related references: PMID:40044865, PMID:40101766
• MPC1 localizes to the mitochondrial inner membrane and contains transmembrane helices, consistent with its transport function.
• Post-translational modifications include acetylation, as identified through proteomics approaches.
• Disease variants in MPC1 have been reported, linking the protein to metabolic disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50–109 of human MPC1, suggesting the antibody likely recognizes an epitope within this region.
• For Western blot, MPC1 has a predicted molecular weight of ~12 kDa; consider using 0.2 µm membranes and carefully optimized transfer times for small proteins.
• In immunoprecipitation experiments, validate antibody performance in the relevant sample system, as polyclonal antibodies may exhibit lot-to-lot variability.
• Cross-reactivity with mouse and rat MPC1 indicates potential utility in rodent models; confirm epitope sequence homology if precise reactivity is critical.
• For ELISA, suitable detection conditions (e.g., coating buffer, blocking agents) should be optimized based on the assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting covering every stage from concept to clinic. For mitochondrial metabolism and pyruvate transport research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Our role as a supply partner ensures reliable procurement of high-quality reagents, while our technical team supports your development pipeline with tailored solutions.