Mouse Anti-Rabbit IgM (Fc) Monoclonal Antibody for Flow Cytometry - P03988

Core Product Specifications and Parameters

Biological Background: IgM Fc Region Function and Localization

• The Ig mu chain C region secreted form (P03988) is a key component of the IgM constant region, providing structural support to the antibody.
• It is a secreted glycoprotein, as annotated in UniProt, consistent with its presence in serum as part of pentameric IgM.
• The protein features disulfide bonds that stabilize the immunoglobulin fold and mediate heavy chain association.
• It contains immunoglobulin-like domains, placing it in the immunoglobulin superfamily.
• Alternative splicing generates different isoforms of the mu chain, enabling both membrane-bound and secreted forms of IgM (this entry represents the secreted form).

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , a bright and photostable green fluorophore, making it immediately ready for flow cytometry without a secondary antibody.
• The immunogen corresponds to the Fc region (amino acids 107-458) of rabbit IgM, ensuring specific recognition of the heavy chain constant domain and reducing cross-reactivity with other rabbit immunoglobulins or light chains.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your cell type; a starting range of 0.1-1 µg per million cells is common for surface staining.
• Include appropriate controls, such as an isotype control antibody and a viability dye, to distinguish specific from non-specific binding.
• If staining secreted or serum IgM, consider the potential for high background; blocking with normal rabbit serum may improve specificity.
• Avoid repeated freeze-thaw cycles; store at 4°C protected from light for short-term use, or aliquot and store at -20°C for long-term storage.
• Validate specificity by testing on rabbit splenocytes or B cell lines, and confirm lack of reactivity with IgM from other species listed as non-cross reactive.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and research labs with a comprehensive range of IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this anti-rabbit IgM Fc conjugate. With expertise in immunoglobulin targets and rare reagent sourcing, CamelBio supports customers from concept to clinic. Explore our catalog for bulk ancillary reagents and customization options to accelerate your assay development.