Mouse anti-Human CD56 (NCAM1) Monoclonal Antibody for Flow Cytometry - P13591

Core Product Specifications and Parameters

Biological Background: CD56 (NCAM1) Function and Localization

• Neural cell adhesion molecule 1 (NCAM1), also known as CD56, belongs to the immunoglobulin superfamily and mediates cell-cell adhesion.
• Plays a critical role in neuron-neuron adhesion, promoting neurite fasciculation and outgrowth during nervous system development.
• Multiple isoforms produced by alternative splicing exhibit molecular weights from approximately 40 kDa to over 95 kDa, including transmembrane and GPI-anchored variants.
• Acts as a receptor for microbial infection: mediates cellular entry of rabies virus and Zika virus.
• Subcellular localization: primarily localized to the plasma membrane; also detected as secreted forms and in lipid rafts via GPI anchoring.
• Post-translational modifications include N-linked glycosylation and phosphorylation, regulating its adhesive properties and signaling.

Experimental Guidance and Technical Tips

• This antibody was raised against recombinant full-length human CD56 protein, making it suitable for detecting native CD56 in flow cytometry.
• For optimal performance, titrate the antibody for each cell type and include appropriate isotype controls to assess non-specific binding.
• Since CD56 is expressed on NK cells and some neurons, validate in the relevant sample system and consider co-staining with lineage-specific markers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, supporting diagnostic manufacturers with validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-CD56 monoclonal antibody exemplifies our commitment to reliable immunophenotyping tools for neuroscience and infectious disease research. For rare target sourcing or custom antibody development, our technical team is ready to assist from concept to clinic.