Mouse anti-Human CD3 Monoclonal Antibody for FC and In Vitro T Cell Stimulation - P07766

Core Product Specifications and Parameters

Biological Background: CD3 Function and Localization

• CD3E is a subunit of the TCR-CD3 complex essential for adaptive immunity and transmits activation signals across the T-cell membrane upon antigen recognition. Related references: PMID:15294938 PMID:15546002 PMID:2470098 PMID:40592325
• The cytoplasmic domain of CD3E contains immunoreceptor tyrosine-based activation motifs (ITAMs) that are phosphorylated by LCK and FYN Src kinases, initiating downstream signaling pathways. Related references: PMID:2470098 PMID:40592325
• CD3E also regulates TCR internalization and cell surface down-regulation via endocytosis sequences in its cytosolic tail. Related references: PMID:10384095 PMID:26507128
• In addition to its coreceptor role, CD3E serves as a receptor for ITPRIPL1, which inhibits T-cell activation by promoting NCK1 interaction, thereby blocking CD3E-ZAP70 binding and downstream ERK/NFkB signaling and calcium influx. Related references: PMID:38614099 PMID:12110186
• CD3E is required for correct T-cell development (by similarity) and localizes to the cell membrane as a single-pass type I membrane protein.

Experimental Guidance and Technical Tips

• For flow cytometry (FC), titrate the antibody to determine the optimal concentration for your sample and include appropriate isotype controls to distinguish specific binding.
• For in vitro T-cell stimulation/activation, ensure sterile working conditions and use an optimized protocol; the antibody can be employed in plate-bound or soluble formats, depending on the experimental design.
• Since CD3E is expressed on T cells, validate the antibody's reactivity in your specific T-cell subset or activation state using relevant controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—supporting every stage from concept to clinic. This mouse anti-human CD3 monoclonal antibody is part of a curated portfolio for immunology and T-cell research. Through validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing, CamelBio streamlines your supply chain for diagnostic development.