Mouse Anti-Human CD1a Monoclonal Antibody for Flow Cytometry - P06126

Core Product Specifications and Parameters

Biological Background: CD1a Function and Localization

• CD1a is a T-cell surface glycoprotein and member of the CD1 family of antigen-presenting molecules, structurally related to MHC class I, specialized in lipid and glycolipid antigen presentation.
• It serves a key role in adaptive immunity by binding both self and non-self lipid antigens and presenting them to T-cell receptors on natural killer T cells, bridging innate and adaptive responses.
• Subcellular localization includes the cell membrane, where it is enriched in membrane rafts, and endosome membranes, which are critical for antigen loading and trafficking.
• CD1a is predominantly expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, and certain T-cell leukemias, with broader tissue distribution noted.
• The protein features an extracellular immunoglobulin-like domain, a transmembrane helix, and is post-translationally modified by N-linked glycosylation and a conserved disulfide bond that stabilizes its structure.
• UniProt annotations highlight keywords such as Adaptive immunity, Glycoprotein, Immunoglobulin domain, and Membrane, underscoring its immune receptor function.

Experimental Guidance and Technical Tips

• Since this product is a directly conjugated antibody, it is ready for flow cytometry without the need for secondary staining; ensure minimal light exposure to preserve fluorophore stability.
• The immunogen is a synthetic peptide derived from human CD1a; thus, the antibody likely recognizes a linear epitope, which may be useful for both native and fixed/permeabilized cell preparations, but confirm empirically.
• For flow cytometry, titrate the antibody in the relevant sample system (e.g., human peripheral blood mononuclear cells or thymocyte suspensions) to determine the optimal concentration that gives maximal signal-to-noise ratio.
• Include appropriate isotype control antibodies (mouse IgG conjugated to ) and viability dyes to gate out dead cells and non-specific binding.
• The reported molecular weight of CD1a is approximately 37 kDa (or 49 kDa glycosylated), but detection in flow cytometry is cell surface-based; no protein weight detection is involved.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a seamless supply of IVD raw materials, including validated monoclonal antibodies like this CD1a reagent. Our portfolio spans optimized antibodies, bulk ancillary reagents, and rare target sourcing to accelerate immunological assay development. Whether you need antibody pairs, recombinant proteins, or custom-sourcing for lipid presentation studies, CamelBio provides reliable, one-stop support from concept to clinic.