Mouse anti-Human CD15/SSEA-1 Monoclonal Antibody for Flow Cytometry - P22083

Core Product Specifications and Parameters

Biological Background: CD15/SSEA-1 Function and Localization

• The CD15/SSEA-1 epitope corresponds to the Lewis X (LeX) glycan structure, which is synthesized by alpha-(1,3)-fucosyltransferase 4 (FUT4) encoded by the FUT4 gene.
• FUT4 catalyzes the transfer of fucose to N-acetylglucosamine of type 2 lactosamine chains on glycoproteins, forming the CD15/SSEA-1 antigen that mediates cell adhesion and signaling.
  Related references:
  PMID:1702034 PMID:1716630 PMID:29593094
• As a member of the fucosyltransferase family, FUT4 also generates sialyl Lewis X and 6-sulfo sialyl Lewis X epitopes, which serve as selectin ligands crucial for leukocyte trafficking.
  Related references:
  PMID:1716630 PMID:29593094
• In certain cell types, FUT4 can fucosylate internal LacNAc units to produce VIM-2 antigen, another selectin recognition motif.
  Related references:
  PMID:11278338 PMID:1716630
• FUT4 localizes to the Golgi apparatus membrane where it performs its glycosylation function.
• The enzyme is a single-pass type II membrane protein with a short cytosolic tail, a transmembrane helix, and a catalytic domain within the Golgi lumen.
• CD15/SSEA-1 is expressed on human myeloid cells, promyelocytes, and lymphoid populations, as well as on embryonic stem cells and some cancer stem cells, serving as a common immunological and developmental marker.
• Alternative initiation produces multiple isoforms of FUT4, and the protein is subject to N-glycosylation, which may affect its activity and stability.

Experimental Guidance and Technical Tips

• The antibody is generated against a synthetic peptide derived from human CD15/SSEA-1. Given its peptide immunogen, it may recognize the protein backbone of FUT4 or the glycan epitope depending on the epitope design.
• For flow cytometry, ensure appropriate isotype controls and titrate the antibody for optimal staining. Consider using EDTA or enzyme-free cell dissociation for live-cell staining to preserve surface epitopes.
• CD15/SSEA-1 is a carbohydrate epitope present on multiple glycoproteins; thus, staining patterns may reflect broader glycan distribution beyond FUT4-specific expression. Validate specificity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For CD15/SSEA-1 research in stem cell biology, immunophenotyping, and cancer diagnostics, we provide reliable reagents like this -conjugated monoclonal antibody to accelerate your assay development. Our supply capabilities span from rare targets to production-scale quantities, ensuring you get the right materials at every stage.